Journal: Cell Metabolism

Article Title: Nutrient-sensing AgRP neurons relay control of liver autophagy during energy deprivation

doi: 10.1016/j.cmet.2023.03.019

Figure Lengend Snippet:

Article Snippet: Sections were then mounted with DAPI using Vectashield Antifade Mounting Medium (Vector Laboratories), covered using a coverslip and stored at 4°C in the dark.

Techniques: Virus, Plasmid Preparation, Recombinant, Protease Inhibitor, Western Blot, Blocking Assay, In Vitro, In Vivo, Enzyme-linked Immunosorbent Assay, Isolation, Bicinchoninic Acid Protein Assay, Reverse Transcription, RNAscope, Multiplex Assay, Software, Microscopy, Mass Spectrometry, Liquid Chromatography, Chromatography

Journal: BMC microbiology

Article Title: Moonlighting proteins are variably exposed at the cell surfaces of Candida glabrata, Candida parapsilosis and Candida tropicalis under certain growth conditions.

doi: 10.1186/s12866-019-1524-5

Figure Lengend Snippet: Fig. 1 Distribution of surface-exposed fungal proteins and their division into major functional groups. C. glabrata, C. parapsilosis and C. tropicalis cells were cultured aerobically in different growth media at 37 °C for 16 h (DS, an amino acid-based, chemically defined liquid synthetic medium; AS, artificial saliva, VS, vagina-simulative medium, and AU, artificial urine) or cultured for 72 h in YPD medium under anaerobic conditions (AN). Surface-exposed proteins were identified by cell surface shaving with trypsin followed by liquid chromatography coupled to tandem mass spectrometry. The protein classifications were made on the basis of the Gene Ontology (GO) annotations (molecular function and involvement in similar cellular processes) from the Candida Genome Database (CGD) and Saccharomyces Genome Database (SGD)

Article Snippet: Cells were cultured in YPD medium (1% yeast extract, 2% soybean peptone and 2% glucose, pH 6.0; Sigma, St. Louis, MO) for 16 h at 30 °C, and 5 x 108 cell aliquots were inoculated into 20 ml of various growth media and further cultured aerobically for 16 h or anaerobically for 72 h at 37 °C on an orbital rotary shaker MaxQ 6000 (170 rpm) (Thermo Fisher Scientific, Waltham, MA).

Techniques: Functional Assay, Cell Culture, Liquid Chromatography, Mass Spectrometry